锝-99m标记亚马逊利什曼原虫抗无鞭毛体多克隆抗体。
Technetium-99m labeling anti-amastigote polyclonal antibodies of Leishmania amazonensis.
作者信息
Araujo J G V C, Toledo V P C P, Guimarães T M P D, Bernardo-Filho M, Simal C J R, Mota L G, Diniz S O F, Cardoso V N
机构信息
Laboratório de Radioisótopos, Faculdade de Farmácia, UFMG, Brazil.
出版信息
Nucl Med Biol. 2002 May;29(4):405-11. doi: 10.1016/s0969-8051(02)00295-0.
Anti-amastigote polyclonal antibody (IgG) was incubated with solutions of stannous chloride and sodium borohidride. After that, 3.7 MBq of technetium-99m (99mTc) was added. A labeling yield of the antibody about 84% was obtained. After filtration of 99mTc-IgG, the radiochemical purity increased from 84 to 95%. The labeling of IgG with 99mTc did not modify the immunoreactivity of the antibody, since it was able to identify in vitro and in vivo the specific antigen of Leishmania amazonensis.
抗无鞭毛体多克隆抗体（IgG）与氯化亚锡和硼氢化钠溶液孵育。之后，加入3.7兆贝可的锝-99m（99mTc）。获得了约84%的抗体标记率。对99mTc-IgG进行过滤后，放射化学纯度从84%提高到95%。用99mTc标记IgG并未改变抗体的免疫反应性，因为它能够在体外和体内识别亚马逊利什曼原虫的特异性抗原。
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