Zhang Xiuqing, Yang Huanming, Yu Jun, Chen Cong, Zhang Guangyu, Bao Jingyue, Du Yutao, Kibukawa Miho, Li Zhijie, Wang Jun, Hu Songnian, Dong Wei, Wang Jian, Gregersen Niels, Niebuhr Erik, Bolund Lars
Human Genome Center, Institute of Genetics, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.
Gene. 2002 Apr 17;288(1-2):9-18. doi: 10.1016/s0378-1119(02)00470-5.
Nucleoporin 155 (Nup155) is a major component of the nuclear pore complex (NPC) involved in cellular nucleo-cytoplasmic transport. We have acquired the complete sequence and interpreted the genomic organization of the Nup155 orthologos from human (Homo sapiens) and pufferfish (Fugu rubripes), which are approximately 80 and 8 kb in length, respectively. The human gene is ubiquitously expressed in many tissues analyzed and has two major transcript variants, resulted from an alternative usage of the 5' cryptic or consensus splice donor in intron 1 and two polyadenylation signals. We have also cloned DNA complementary to RNAs of the Nup155 orthologs from Fugu and mouse. Comparative analysis of the Nup155 orthologs in many species, including H. sapiens, Mus musculus, Rattus norvegicus, F. rubripes, Arabidopsis thaliana, Drosophila melanogaster, and Saccharomyces cerevisiae, has revealed two paralogs in S. cerevisiae but only a single gene with increasing number of introns in more complex organisms. The amino acid sequences of the Nup155 orthologos are highly conserved in the evolution of eukaryotes. Different gene orders in the human and Fugu genomic regions harboring the Nup155 orthologs advocate cautious interpretation of synteny in comparative genomic analysis even within the vertebrate lineage.
核孔蛋白155（Nup155）是核孔复合体（NPC）的主要组成部分，参与细胞的核质运输。我们已获得人类（智人）和河豚（红鳍东方鲀）Nup155直系同源基因的完整序列，并解读了其基因组结构，其长度分别约为80 kb和8 kb。人类基因在分析的许多组织中均有广泛表达，有两个主要转录变体，这是由于内含子1中5'隐蔽或共有剪接供体的选择性使用以及两个聚腺苷酸化信号所致。我们还克隆了与河豚和小鼠Nup155直系同源基因的RNA互补的DNA。对包括智人、小家鼠、褐家鼠、红鳍东方鲀、拟南芥、黑腹果蝇和酿酒酵母在内的许多物种的Nup155直系同源基因进行比较分析，发现酿酒酵母中有两个旁系同源基因，而在更复杂的生物体中只有一个内含子数量不断增加的单一基因。Nup155直系同源基因的氨基酸序列在真核生物进化过程中高度保守。人类和河豚基因组中含有Nup155直系同源基因的区域中不同的基因顺序表明，即使在脊椎动物谱系中，在比较基因组分析中对同线性的解读也需谨慎。
