Esteve M J, Frígola A, Rodrigo M C, Rodrigo M
Nutrition and Food Chemistry, Faculty of Pharmacy, University of Valencia, Avda. Vicent Andrés Estellés, s/n. E-46100 Burjassot, Valencia, Spain.
Food Addit Contam. 2002 Jun;19(6):519-23. doi: 10.1080/02652030210125128.
The determination of diacetyl permits the detection of microbial growth in the processing of citrus fruit before the appearance of other organoleptic, chemical or microbiological changes. It also makes it possible to detect a break in the cold chain during distribution and sale. The study proposed a polarographic method for the determination of diacetyl that allowed routine analysis with the aim of detecting possible contamination in the citrus juice manufacturing chain (orange and orange-carrot). The analytical performance of the method in terms of a linearity from 0 to 960 microg ml(-1), a recovery of 97 to 98%, a precision of 3.2 to 4.8%, and a sensitivity of 0.2 ng ml(-1) for juices indicated the robustness of the method. The same method was also applied to butter and yoghurt with detection limits of and 0.4 ng g(-1).
双乙酰的测定能够在柑橘类水果加工过程中，在出现其他感官、化学或微生物变化之前检测到微生物的生长。它还能在分销和销售过程中检测冷链的中断情况。该研究提出了一种测定双乙酰的极谱法，以便进行常规分析，目的是检测柑橘汁生产链（橙汁和橙胡萝卜汁）中可能存在的污染。该方法在0至960微克/毫升的线性范围内、回收率为97%至98%、精密度为3.2%至4.8%以及对果汁的灵敏度为0.2纳克/毫升等方面的分析性能表明了该方法的稳健性。同样的方法也应用于黄油和酸奶，检测限分别为[此处原文缺失黄油的检测限数据]和0.4纳克/克。
