Huang Ju, Hao Li, Liu Su, Li Lin, Zhang Wen-Xia, Dai Zhuo-Hua
College of Life Sciences, Peking University, Beijing 100871, China.
Yi Chuan Xue Bao. 2002 May;29(5):417-23.
In order to investigate the phylogenetic position of Chinese endemic Drosophila curviceps species subgroup within the D. immigrans group on DNA molecular level, the ITS1 region of ribosomal DNA and part of Adh gene for 12 species represented all the five subgroups in D. immigrans group were sequenced. Molecular phylogenetic trees were constructed by using parsimony and neighbor-joining methods based on ITS1, Adh and combined sequences. In the molecular trees, species of the D. curviceps subgroup cluster together, strongly supporting establishment of a separating subgroup suggested in an initial morphologic study. This subgroup is the sister taxa of the D. quadrilineata subgroup. They are the younger subgroups, splitting about 3.4 Mya. The D. hypocausta subgroup is located at the base of the molecular tree, diverged first in this group about 9.2 Mya. Taking the geographic distribution into account, we can conclude that D. curviceps subgroup originated from tropic zones, agreeing with the previous morphologic and biogeographic suggestions. The position of D. neohypocausta was also discussed in this report and it seems that it is closer to the D. immigrans subgroup than to the D. hypocausta subgroup.
为了在DNA分子水平上研究中国特有种弯翅果蝇种组在迁飞果蝇种组中的系统发育位置，对代表迁飞果蝇种组中所有五个种组的12个物种的核糖体DNA的ITS1区域和部分乙醇脱氢酶（Adh）基因进行了测序。基于ITS1、Adh及联合序列，采用简约法和邻接法构建了分子系统发育树。在分子树中，弯翅果蝇种组的物种聚集在一起，有力地支持了最初形态学研究中提出的建立一个独立种组的观点。该种组是四线果蝇种组的姐妹类群。它们是较年轻的种组，大约在340万年前分化。低卡斯塔果蝇种组位于分子树的基部，在该种组中约在920万年前首先分化。综合地理分布情况，我们可以得出结论，弯翅果蝇种组起源于热带地区，这与之前的形态学和生物地理学观点一致。本报告还讨论了新低卡斯塔果蝇的位置，它似乎更接近迁飞果蝇种组而非低卡斯塔果蝇种组。
