Shan Xue-Song, Zhang Yuan, Li Ning
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China.
Yi Chuan Xue Bao. 2002 May;29(5):430-3.
The amplified fragment length polymorphisms of 7 microsatellite DNA loci linked with weaver gene according to the comparative map of mouse and cattle were detected in 178 Holstein cattle. Results showed that there were 4 and 6 alleles at BM6438 and BMS2321 loci respectively. Two alleles at BMS4020 locus and 5 alleles at BMS711 locus were detected. Two alleles at INRA117, 3 alleles at CA095 and 3 alleles at TGLA116 locus were found. Among these 7 microsatellite loci, BM6438, BMS711 and BMS2321 were high polymorphic loci, and PIC(polymorphism information content) was 0.58, 0.67 and 0.67 respectively. PIC of the moderate polymorphic locus TGLA116 was 0.45. BMS4020, INRA117 and CA095 were low polymorphic loci with PIC of 0.06, 0.05 and 0.05 respectively. Effects of BM6438, BMS2321, BMS711 and TGLA116 on milk production traits were analyzed with PROC GLM of SAS. Results showed that the four loci had no significant effect on milk yield of Holstein cattle, effect of BM6438 and BMS711 on milk composition was not significant either(P > 0.05). BMS2321 had a significant effect on protein yield and protein percentage(P < 0.01). The effect of TGLA116 on protein yield and protein percentage reached a significant level at P < 0.05.
根据小鼠和牛的比较图谱，在178头荷斯坦奶牛中检测了与韦弗基因相关的7个微卫星DNA位点的扩增片段长度多态性。结果表明，BM6438和BMS2321位点分别有4个和6个等位基因。检测到BMS4020位点有2个等位基因，BMS711位点有5个等位基因。发现INRA117有2个等位基因，CA095有3个等位基因，TGLA116位点有3个等位基因。在这7个微卫星位点中，BM6438、BMS711和BMS2321是高度多态性位点，多态性信息含量（PIC）分别为0.58、0.67和0.67。中度多态性位点TGLA116的PIC为0.45。BMS4020、INRA117和CA095是低度多态性位点，PIC分别为0.06、0.05和0.05。用SAS的PROC GLM分析了BM6438、BMS2321、BMS711和TGLA116对产奶性状的影响。结果表明，这4个位点对荷斯坦奶牛的产奶量没有显著影响，BM6438和BMS711对牛奶成分的影响也不显著（P>0.05）。BMS2321对蛋白质产量和蛋白质百分比有显著影响（P<0.01）。TGLA116对蛋白质产量和蛋白质百分比的影响在P<0.05时达到显著水平。
