Kagan-Zur V, Lips S H
Eur J Biochem. 1975 Nov 1;59(1):17-23. doi: 10.1111/j.1432-1033.1975.tb02419.x.
Homogenates of dark-pretreated leaves yield two particulate fractions in density gradient centrifugation: one contains chlorophyll (chloroplasts) while a second fraction contains ribulose-1, 5-bisphophate carboxylase, NADP-dependent glyceraldehyde-3-phosphate dehydrogenase and catalase. Addition of a microbody-rich pellet to chloroplasts isolated from dark-pretreated plants largely enhances both oxygen evolution and CO2-fixation into organic compounds. The pathway of CO2 reduction may be part of a membrane system which, under suitable conditions, may separate from the chloroplast as a distinct cytoplasmic entity, having physical properties similar to those of microbodies.
一个含有叶绿素(叶绿体),而另一个组分含有核酮糖-1,5-二磷酸羧化酶、NADP 依赖性甘油醛-3-磷酸脱氢酶和过氧化氢酶。将富含微体的沉淀添加到从暗预处理植物中分离出的叶绿体中,可大大增强氧气释放以及二氧化碳固定为有机化合物的能力。二氧化碳还原途径可能是膜系统的一部分,在合适的条件下,该膜系统可能作为一个独特的细胞质实体与叶绿体分离,其物理性质与微体相似。