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光合碳还原循环中酶的细胞内定位研究。

Studies on the intracellular location of enzymes of the photosynthetic carbon-reduction cycle.

作者信息

Kagan-Zur V, Lips S H

出版信息

Eur J Biochem. 1975 Nov 1;59(1):17-23. doi: 10.1111/j.1432-1033.1975.tb02419.x.

DOI:10.1111/j.1432-1033.1975.tb02419.x
PMID:1204606
Abstract

Homogenates of dark-pretreated leaves yield two particulate fractions in density gradient centrifugation: one contains chlorophyll (chloroplasts) while a second fraction contains ribulose-1, 5-bisphophate carboxylase, NADP-dependent glyceraldehyde-3-phosphate dehydrogenase and catalase. Addition of a microbody-rich pellet to chloroplasts isolated from dark-pretreated plants largely enhances both oxygen evolution and CO2-fixation into organic compounds. The pathway of CO2 reduction may be part of a membrane system which, under suitable conditions, may separate from the chloroplast as a distinct cytoplasmic entity, having physical properties similar to those of microbodies.

摘要

经过暗预处理的叶片匀浆在密度梯度离心中产生两个颗粒组分

一个含有叶绿素(叶绿体),而另一个组分含有核酮糖-1,5-二磷酸羧化酶、NADP 依赖性甘油醛-3-磷酸脱氢酶和过氧化氢酶。将富含微体的沉淀添加到从暗预处理植物中分离出的叶绿体中,可大大增强氧气释放以及二氧化碳固定为有机化合物的能力。二氧化碳还原途径可能是膜系统的一部分,在合适的条件下,该膜系统可能作为一个独特的细胞质实体与叶绿体分离,其物理性质与微体相似。

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引用本文的文献

1
Chloroplast envelopes as affected by light or dark pretreatment of pea plants.叶绿体被膜受豌豆植株预先照光或照暗的影响。
Planta. 1980 Oct;149(5):427-32. doi: 10.1007/BF00385743.
2
Specific deficit in the synthesis of 6-sulfoquinovsyl diglyceride in Chlorella pyrenoidosa.小球藻中6-磺基喹诺糖基二酰甘油合成的特异性缺陷。
J Bacteriol. 1977 Jan;129(1):516-24. doi: 10.1128/jb.129.1.516-524.1977.