Subirá Dolores, Castañón Susana, Aceituno Esther, Hernández Jaime, Jiménez-Garófano Carmen, Jiménez Antonio, Jiménez Ana María, Román Alejandro, Orfao Alberto
Department of Haematology, Jiménez Díaz Foundation, Madrid, Spain.
Am J Clin Pathol. 2002 Jun;117(6):952-8. doi: 10.1309/123P-CE6V-WYAK-BB1F.
Low volume and few cells have hampered the use of flow cytometry for studying cerebrospinal fluid (CSF) in routine clinical practice, although information about the cellular phenotypes present in this type of sample is of great value in many diseases. We developed a novel flow cytometric strategy capable of identifying total CSF T lymphocytes and the CD4+ subset, even in CSF samples with as few as 1 leukocyte per 3 microL of sample. We also showed that identification of CD8+ T cells could be achieved in most samples, while B lymphocytes are detectable only in samples with more than 5 cells per microliter. These findings demonstrate the reliability of this method to improve the diagnostic accuracy of classic cytologic studies in many neurologic disorders.
样本量少且细胞数量稀少阻碍了流式细胞术在常规临床实践中用于研究脑脊液（CSF），尽管关于这类样本中细胞表型的信息在许多疾病中具有重要价值。我们开发了一种新型流式细胞术策略，即使在每3微升样本中仅有1个白细胞的脑脊液样本中，也能够识别总脑脊液T淋巴细胞和CD4 +亚群。我们还表明，大多数样本中可以实现CD8 + T细胞的识别，而B淋巴细胞仅在每微升有超过5个细胞的样本中才可检测到。这些发现证明了该方法在提高许多神经系统疾病经典细胞学研究诊断准确性方面的可靠性。
