Melrose T R, Brown C G
Res Vet Sci. 1979 Nov;27(3):379-81.
Blood from calves infected with Theileria annulata and T parva was freed from host cell elements and the piroplasms liberated from the red cells by ammonium chloride lysis. Lysates of the purified piroplasms and control host cell material were examined electrophoretically for several enzymes. Zymograms stained for glucose phosphate isomerase showed distinct differences between the host cell enzyme pattern and parasite enzyme patterns. The isoenzyme pattern of T annulata piroplasms differed from the isoenzyme pattern of T parva piroplasms.
从感染环形泰勒虫和微小泰勒虫的小牛采集血液，通过氯化铵裂解去除宿主细胞成分，使红细胞中的梨形虫体释放出来。对纯化的梨形虫体裂解物和对照宿主细胞材料进行了几种酶的电泳分析。磷酸葡萄糖异构酶染色的酶谱显示，宿主细胞酶谱与寄生虫酶谱之间存在明显差异。环形泰勒虫梨形虫体的同工酶谱与微小泰勒虫梨形虫体的同工酶谱不同。
