重组葡激酶对中国实验用小型猪冠状动脉血栓形成的影响。

Effects of recombinant staphylokinase on coronary thrombosis in Chinese experimental miniature swine.

作者信息

Liu Jian-Xun, Shang Xiao-Hong, Fu Jian-Hua, Li Xin-Zhi, Wang Gang

机构信息

Department of Basic Medicine Science, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China.

出版信息

Acta Pharmacol Sin. 2002 Jun;23(6):509-15.

PMID:12060524

Abstract

AIM

To study the effects of recombinant staphylokinase (r-Sak) on coronary thrombosis, cardiac ischemia, and myocardial infarction in Chinese experimental miniature swine.

METHODS

Endarterium was injuried and coronary thrombi were formed gradually through direct electrical stimulation on the coronary artery of Chinese experimental miniature swine. Effects of r-Sak in vitro were studied through coronary pathological section, microkinematography, multi media graphic analysis, epicardial electrogram mapping, myocardium histo chemical stain, serum creatine phosphokinase (CPK), and hemorheology.

RESULTS

r-Sak showed a remarkable effect on coronary thrombus. Compared w ith the control group, the two dosage groups, r-Sak 0.45 mg/kg and 1.35 mg/kg, reduced the transverse section area of coronary thrombus (P<0.01), lessened the degree and range of cardiac ischemia (P<0.01), decreased the myocardial infarction area (P<0.01), the activity of CPK and blood viscosity (P<0.05), restrained platelet adhesion and aggregation, and reduced fibrinogen concentration (P<0.05).

CONCLUSION

r-Sak could dissolve coronary thrombus obviously and lessen the pathologic reaction of myocardium.

摘要

目的

研究重组葡激酶（r-Sak）对中国实验用小型猪冠状动脉血栓形成、心肌缺血及心肌梗死的影响。

方法

通过直接电刺激中国实验用小型猪冠状动脉造成内膜损伤并逐渐形成冠状动脉血栓。通过冠状动脉病理切片、显微动态成像、多媒体图像分析、心外膜心电图标测、心肌组织化学染色、血清肌酸磷酸激酶（CPK）及血液流变学等方法研究r-Sak的体外作用。

结果

r-Sak对冠状动脉血栓有显著作用。与对照组相比，r-Sak 0.45 mg/kg和1.35 mg/kg两个剂量组均减小了冠状动脉血栓横截面积（P<0.01），减轻了心肌缺血程度和范围（P<0.01），减小了心肌梗死面积（P<0.01），降低了CPK活性和血液黏度（P<0.05），抑制了血小板黏附和聚集，并降低了纤维蛋白原浓度（P<0.05）。