Liu Jian-xun, Li Xin-zhi, Cong Wei-hong, Fu Jian-hua, Shang Xiao-hong
Fundamental Medical Sciences Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China.
Chin J Integr Med. 2007 Sep;13(3):206-10. doi: 10.1007/s11655-007-0206-6.
To investigate the effects of Corocalm (shuguan capsule) on acute myocardial ischemia in anesthetized dogs and its possible therapeutic mechanism.
The acute ischemia model was established by ligating the left anterior descending (LAD) artery. Twenty-five dogs were randomly divided into 5 groups (5 dogs in each group): the control group (treated with normal saline 3 mL/kg), the refined Guanxin Capsule group (GXC 200 mg/kg), high and low dose Corocalm groups (48.5 mg/kg for low dose group and 194.0 mg/kg for high dose group) and the Diltiazem group (5 mg/kg). The animals were treated via a single duodenal administration after the model was established. The experiments used epicardial electrocardiogram (EECG) to measure the scope and degree of myocardial ischemia. Simultaneously, the coronary blood flow (CBF) and serum activity levels of creatine phosphokinase (CK) and lactate dehydrogenase (LDH) were measured by electromagnetic flow meter and automatic biochemical analyzer respectively. The plasma endothelin (ET) content was quantified by radioimmunoassay.
Corocalm (48.5 mg/kg and 194.0 mg/kg) significantly decreased the degree and scope of myocardial ischemia, reduced the infarct area, markedly increased the CBF, and inhibited the increase of CK and LDH activities and ET levels induced by myocardial ischemia/infarction.
Corocalm could improve the state of acute myocardial ischemia and infarction in dogs. The mechanism of action might be correlated to increasing CBF, inhibiting CK and LDH activities and preventing ET release.
研究舒心胶囊对麻醉犬急性心肌缺血的影响及其可能的治疗机制。
通过结扎左冠状动脉前降支建立急性缺血模型。25只犬随机分为5组（每组5只）：对照组（给予3 mL/kg生理盐水）、精制冠心胶囊组（GXC 200 mg/kg）、舒心胶囊高、低剂量组（低剂量组48.5 mg/kg，高剂量组194.0 mg/kg）和地尔硫䓬组（5 mg/kg）。模型建立后经十二指肠单次给药。实验采用体表心电图（EECG）测量心肌缺血的范围和程度。同时，分别用电磁流量计和自动生化分析仪测量冠状动脉血流量（CBF）以及肌酸磷酸激酶（CK）和乳酸脱氢酶（LDH）的血清活性水平。采用放射免疫分析法测定血浆内皮素（ET）含量。
舒心胶囊（48.5 mg/kg和194.0 mg/kg）能显著降低心肌缺血的程度和范围，减少梗死面积，明显增加CBF，并抑制心肌缺血/梗死所致的CK和LDH活性及ET水平升高。
舒心胶囊可改善犬急性心肌缺血和梗死状态。其作用机制可能与增加CBF、抑制CK和LDH活性以及阻止ET释放有关。
