Broadwell R D
J Comp Neurol. 1975 Dec 15;164(4):389-409. doi: 10.1002/cne.901640402.
The efferent connections of the anterior olfactory nucleus in the female albino rabbit have been studied using the autoradiographic and horseradish peroxidase methods for tracing axonal pathways. Following a unilateral injection of 3H-leucine into the olfactory peduncle, radioactively labeled efferent projections from the anterior olfactory nucleus were traced into all layers of the ipsilateral main olfactory bulb beneath the olfactory nerve layer and through the ipsilateral anterior limb of the anterior commissure and plexiform layer of the medial side of the cerebral hemisphere to the deep half of the plexiform (IB) and pyramidal cell (II) layers of the prepyriform cortex, the tenia tecta, and the entire surface of the olfactory tubercle. Labeled projections crossing the midline within the anterior commissure were followed to the layers IB and II of the contralateral anterior prepyriform cortex and pars externa, pars lateralis, and pars dorsalis of the anterior olfactory nucleus, and through the periventricular layer of the olfactory peduncle to all layers of the main olfactory bulb beneath the olfactory nerve layer. No well-defined labeled projection was traced to the contralateral accessory olfactory bulb. Evidence for possible anterior olfactory nucleus and/or prepyriform cortical projections to the ipsilateral paleocortical half of the claustrum, horizontal limb of the nucleus of the diagonal band, the posterior lateral hypothalamus at the level of the mammillary complex, and to the bed nucleus of the stria terminalis is discussed. Intra-axonal retrograde transport of horseradish peroxidase from axon terminals to parent cell bodies after unilateral injection of the protein into the main olfactory bulb or anterior olfactory nucleus revealed that anterior olfactory nucleus projections to the olfactory bulbs and the contralateral anterior olfactory nucleus arise predominately from the pars externa. The autoradiographic data indicate that the anterior olfactory nucleus projects to olfactory cortical structures which also receive afferent input from the olfactory bulb and that the termination of these projections is complementary to those from the olfactory bulb.
利用放射自显影法和辣根过氧化物酶法追踪轴突通路，对雌性白化兔前嗅核的传出联系进行了研究。在嗅柄单侧注射³H-亮氨酸后，从前嗅核发出的放射性标记传出投射可追踪到嗅神经层下方同侧主嗅球的所有层，并通过前连合的同侧前肢和大脑半球内侧的丛状层，到达梨状前皮质的丛状（IB）层和锥体细胞（II）层的深部、带状层以及嗅结节的整个表面。在前连合内越过中线的标记投射可追踪到对侧梨状前皮质的IB层和II层以及前嗅核的外侧部、外侧部和背侧部，并通过嗅柄的室周层到达嗅神经层下方主嗅球的所有层。未发现明确的标记投射到对侧副嗅球。文中还讨论了前嗅核和/或梨状前皮质可能向同侧屏状核的古皮质半部、斜角带核的水平支、乳头体复合体水平的下丘脑后外侧以及终纹床核投射的证据。在单侧将辣根过氧化物酶注入主嗅球或前嗅核后，从轴突终末向亲代细胞体进行的轴突内逆行运输显示，前嗅核向嗅球和对侧前嗅核的投射主要起源于外侧部。放射自显影数据表明，前嗅核投射到嗅皮质结构，这些结构也接受来自嗅球的传入输入，并且这些投射的终止与来自嗅球的投射互补。
