Szallies Alexander, Kubata Bruno K, Duszenko Michael
Verfügungsgebäude der Universität Tübingen, Auf der Morgenstelle 15, Tübingen, Germany.
FEBS Lett. 2002 Apr 24;517(1-3):144-50. doi: 10.1016/s0014-5793(02)02608-x.
Metacaspases constitute a new group of cysteine proteases homologous to caspases. Heterologous expression of Trypanosoma brucei metacaspase TbMCA4 in the budding yeast Saccharomyces cerevisiae resulted in growth inhibition, mitochondrial dysfunction and clonal death. The metacaspase orthologue of yeast, ScMCA1 (YOR197w), exhibited genetic interaction with WWM1 (YFL010c), which encodes a small WW domain protein. WWM1 overexpression resulted in growth arrest and clonal death, which was suppressed by concomitant overexpression of ScMCA1. GFP-fusion reporters of WWM1, ScMCA1 and TbMCA4 localized to the nucleus. Taken together, we suggest that metacaspases may play a role in nuclear function controlling cellular proliferation coupled to mitochondrial biogenesis.
类半胱天冬酶构成了一组与半胱天冬酶同源的新型半胱氨酸蛋白酶。布氏锥虫类半胱天冬酶TbMCA4在出芽酵母酿酒酵母中的异源表达导致生长抑制、线粒体功能障碍和克隆死亡。酵母的类半胱天冬酶直系同源物ScMCA1（YOR197w）与编码小WW结构域蛋白的WWM1（YFL010c）表现出遗传相互作用。WWM1的过表达导致生长停滞和克隆死亡，而ScMCA1的共过表达可抑制这种情况。WWM1、ScMCA1和TbMCA4的绿色荧光蛋白融合报告基因定位于细胞核。综上所述，我们认为类半胱天冬酶可能在控制细胞增殖与线粒体生物发生相关的核功能中发挥作用。
