McNatty K P, Henderson K M, Sawers R S
J Endocrinol. 1975 Nov;67(2):231-40. doi: 10.1677/joe.0.0670231.
Human granulosa cells with differing steroidogenic potentials were cultured in vitro. The effects of prostaglandin F2alpha (PGF2alpha) and PGE2 on the progesterone output and viability of these cells were investigated. Prostaglandin F2alpha either alone or in combination with LH and FSH inhibited the production of progesterone over a wide range of concentrations (1-8000 ng/ml). However, the inhibitory effect of PGF2alpha was 200 times less effective when the cells were exposed to LH and FSH for 6 days before the addition of the prostaglandin. By contrast PGE2, at concentrations from 1 to 500 ng/ml, markedly stimulated the production of progesterone by granulosa cells, and this was not prevented by the addition of PGF2alpha. The degree of inhibition by PGF2alpha or stimulation by PGE2 was related to the biosynthetic capacity of the cells. These studies suggest that PGF2alpha may act directly on the adenylate cyclase system of human granulosa cells by blocking its activation by LH, and they demonstrate that functional regression of the luteal cell can be induced independently of the blood vascular system.
体外培养具有不同类固醇生成潜能的人颗粒细胞。研究了前列腺素F2α（PGF2α）和前列腺素E2（PGE2）对这些细胞孕酮分泌量和活力的影响。单独的PGF2α或与促黄体生成素（LH）和促卵泡生成素（FSH）联合使用时，在很宽的浓度范围（1 - 8000 ng/ml）内均抑制孕酮的产生。然而，在添加前列腺素之前，若细胞先暴露于LH和FSH 6天，PGF2α的抑制作用效力会降低200倍。相比之下，浓度为1至500 ng/ml的PGE2能显著刺激颗粒细胞产生孕酮，且添加PGF2α并不能阻止这种刺激。PGF2α的抑制程度或PGE2的刺激程度与细胞的生物合成能力有关。这些研究表明，PGF2α可能通过阻断LH对腺苷酸环化酶系统的激活而直接作用于人颗粒细胞的腺苷酸环化酶系统，并且它们证明黄体细胞的功能衰退可独立于血管系统而被诱导。
