Kirikoshi Hiroyuki, Katoh Masaru
Genetics and Cell Biology Section, Genetics Division, National Cancer Center Research Institute, Tokyo 104-0045, Japan.
Int J Oncol. 2002 Jul;21(1):193-6.
We have recently cloned and characterized ST7R (ST7-like, ST7L), WNT3, WNT3A, WNT5B, WNT6, WNT7B, WNT8A, WNT8B, WNT10A, WNT10B, WNT11, WNT14, WNT14B/WNT15, NKD1, NKD2, ARHU/WRCH1, ARHV/WRCH2, and VANGL1/STB2 using bioinformatics, cDNA-PCR and RACE. ST7R is a paralog of tumor suppressor gene ST7 in the human genome. ST7R gene is clustered with WNT2B gene in human chromo-some 1p13 region, while ST7 gene is clustered with WNT2 gene in human chromosome 7q31 region. Multiple ST7R mRNAs (ST7R1-ST7R4) are transcribed due to alternative splicing. ST7R4 is divergent from ST7R1-ST7R3 in the C-terminal region. Here, we investigated expression of ST7R mRNAs in normal human tissues and human cancer. Northern blot analysis with S7S1 probe corresponding to ST7R1, ST7R2 and ST7R3 isoforms detected 4.2 kb ST7R mRNA in various normal tissues, and also large amounts of 2.2-2.4 kb ST7R mRNAs in testis. Northern blot analysis with S7S4 probe corresponding to ST7R4 isoform detected 2.0 kb ST7R mRNA in testis. Expression of ST7R mRNAs in human cancer was next investigated using cDNA-PCR. Although ST7R mRNAs were almost ubiquitously expressed in 7 gastric cancer cell lines, expression levels of ST7R mRNAs were relatively lower in TMK1 cells. ST7R mRNAs were expressed in most cases of primary gastric cancer, and were up-regulated in 2 out of 10 cases of primary gastric cancer. This is the first report on expression analyses on ST7R.
我们最近利用生物信息学、cDNA - PCR和RACE技术克隆并鉴定了ST7R（类ST7，ST7L）、WNT3、WNT3A、WNT5B、WNT6、WNT7B、WNT8A、WNT8B、WNT10A、WNT10B、WNT11、WNT14、WNT14B/WNT15、NKD1、NKD2、ARHU/WRCH1、ARHV/WRCH2以及VANGL1/STB2。ST7R是人类基因组中肿瘤抑制基因ST7的旁系同源基因。ST7R基因与WNT2B基因在人类染色体1p13区域成簇，而ST7基因与WNT2基因在人类染色体7q31区域成簇。由于可变剪接，转录产生了多种ST7R mRNA（ST7R1 - ST7R4）。ST7R4在C末端区域与ST7R1 - ST7R3不同。在此，我们研究了ST7R mRNA在正常人体组织和人类癌症中的表达情况。用对应于ST7R1、ST7R2和ST7R3亚型的S7S1探针进行Northern印迹分析，在各种正常组织中检测到4.2 kb的ST7R mRNA，在睾丸中还检测到大量2.2 - 2.4 kb的ST7R mRNA。用对应于ST7R4亚型的S7S4探针进行Northern印迹分析，在睾丸中检测到2.0 kb的ST7R mRNA。接下来使用cDNA - PCR研究ST7R mRNA在人类癌症中的表达。虽然ST7R mRNA在7种胃癌细胞系中几乎普遍表达，但在TMK1细胞中ST7R mRNA的表达水平相对较低。ST7R mRNA在大多数原发性胃癌病例中表达，在10例原发性胃癌中有2例上调。这是关于ST7R表达分析的首次报道。
