葡萄胎中的倍性与印记。流式细胞术和印记基因产物p57KIP2免疫组化的互补应用以辅助葡萄胎分类。
Ploidy and imprinting in hydatidiform moles. Complementary use of flow cytometry and immunohistochemistry of the imprinted gene product p57KIP2 to assist molar classification.
作者信息
Genest David R, Dorfman David M, Castrillon Diego H
机构信息
Division of Women's and Perinatal Pathology, Hematology Laboratory, Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
出版信息
J Reprod Med. 2002 May;47(5):342-6.
OBJECTIVE
To determine whether the combination of flow cytometry and immunohistochemistry for p57 expression is useful for the pathologic classification of diagnostically challenging hydatidiform moles.
STUDY DESIGN
Six probable hydatidiform moles that were difficult to classify pathologically were reevaluated by histology, flow cytometry and immunohistochemistry for p57.
RESULTS
In all cases, the pathologic diagnoses were easily resolved: two cases were partial moles (triploid, p57 positive), two cases were early complete moles (diploid, p57 negative), and two cases were twin gestations with normal villi (diploid, p57 positive) admixed with villi from a complete hydatidiform mole (diploid, p57 negative).
CONCLUSION
Immunostaining for the paternally imprinted, maternally expressed p57 gene product is a practical and accurate adjunct to flow cytometry in the pathologic classification of hydatidiform moles.
目的
确定流式细胞术与p57表达免疫组化相结合是否有助于对诊断具有挑战性的葡萄胎进行病理分类。
研究设计
对6例病理分类困难的疑似葡萄胎进行组织学、流式细胞术及p57免疫组化重新评估。
结果
在所有病例中，病理诊断均易于明确：2例为部分性葡萄胎（三倍体，p57阳性），2例为早期完全性葡萄胎（二倍体，p57阴性），2例为双胎妊娠，正常绒毛（二倍体，p57阳性）与完全性葡萄胎绒毛（二倍体，p57阴性）混合存在。
结论
对父系印记、母系表达的p57基因产物进行免疫染色，在葡萄胎的病理分类中是一种实用且准确的流式细胞术辅助方法。
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