胶原性结肠炎和炎症性肠病患者结肠黏膜中肥大细胞和巨噬细胞的不同分布。

Different distribution of mast cells and macrophages in colonic mucosa of patients with collagenous colitis and inflammatory bowel disease.

作者信息

Nishida Yoshiyuki, Murase Kunihiko, Isomoto Hajime, Furusu Hisashi, Mizuta Yohei, Riddell Robert H, Kohno Shigeru

机构信息

Second Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, Japan.

出版信息

Hepatogastroenterology. 2002 May-Jun;49(45):678-82.

PMID:12063968

Abstract

BACKGROUND/AIMS: Chronic inflammatory cells in colonic mucosa is a histopathologic feature in patients with collagenous colitis and inflammatory bowel disease. The aim of this study was to compare the distribution of mast cells and macrophages in the colonic mucosa of patients with collagenous colitis, Crohn's disease, and ulcerative colitis.

METHODOLOGY

Patients with histologically confirmed collagenous colitis (n = 13), Crohn's disease (n = 20) or ulcerative colitis (n = 20) and normal control patients (n = 20) were included in this study. Biopsy specimens were obtained from the sigmoid colon of each patient, and immunostained using antibodies to tryptase (AA1) and CD68. The number of mast cells and macrophages located in upper and lower part of the lamina propria was determined.

RESULTS

The number of mast cells in the upper part of lamina propria in patients with collagenous colitis (286 +/- 89/mm2, mean +/- SD), Crohn's disease (330 +/- 84/mm2) and ulcerative colitis (355 +/- 90/mm2), was higher than normal controls (201 +/- 44/mm2). The number of mast cells in the lower part of lamina propria in patients with Crohn's disease (345 +/- 87/mm2) and ulcerative colitis (363 +/- 86/mm2) was higher than collagenous colitis (266 +/- 63/mm2) and normal controls (309 +/- 60/mm2). The number of macrophages in the lower part of lamina propria in patients with Crohn's disease (330 +/- 63/mm2) and ulcerative colitis (301 +/- 60/mm2) was higher than in collagenous colitis (247 +/- 46/mm2) and normal controls (242 +/- 52/mm2), although there were no significant differences in the number of macrophages present in the upper part of the lamina propria among the four groups.

CONCLUSIONS

Our data showed the presence of a different distribution of mast cells and macrophages in collagenous colitis and inflammatory bowel disease, and these suggest that because mucosal mast cells have been implicated in the development of Th2 response collagenous colitis is more of a Th2 type reaction rather than Th1.

摘要

背景/目的：结肠黏膜中的慢性炎症细胞是胶原性结肠炎和炎症性肠病患者的组织病理学特征。本研究的目的是比较胶原性结肠炎、克罗恩病和溃疡性结肠炎患者结肠黏膜中肥大细胞和巨噬细胞的分布情况。

方法

本研究纳入了经组织学确诊的胶原性结肠炎患者（n = 13）、克罗恩病患者（n = 20）、溃疡性结肠炎患者（n = 20）以及正常对照患者（n = 20）。从每位患者的乙状结肠获取活检标本，并用抗胰蛋白酶（AA1）和CD68抗体进行免疫染色。确定位于固有层上部和下部的肥大细胞和巨噬细胞数量。

结果

胶原性结肠炎患者（286±89/mm²，均值±标准差）、克罗恩病患者（330±84/mm²）和溃疡性结肠炎患者（355±90/mm²）固有层上部的肥大细胞数量高于正常对照（201±44/mm²）。克罗恩病患者（345±87/mm²）和溃疡性结肠炎患者（363±86/mm²）固有层下部的肥大细胞数量高于胶原性结肠炎患者（266±63/mm²）和正常对照（309±60/mm²）。克罗恩病患者（330±63/mm²）和溃疡性结肠炎患者（301±60/mm²）固有层下部的巨噬细胞数量高于胶原性结肠炎患者（247±46/mm²）和正常对照（242±52/mm²），尽管四组固有层上部的巨噬细胞数量无显著差异。