与新城疫病毒以及从持续感染的L细胞中分离出的新城疫病毒温度敏感突变体相关的RNA聚合酶的比较。
Comparison of RNA polymerase associated with Newcastle disease virus and a temperature-sensitive mutant of Newcastle disease virus isolated from persistently infected L cells.
作者信息
Stanwick T L, Hallum J V
出版信息
J Virol. 1975 Jan;17(1):68-73. doi: 10.1128/JVI.17.1.68-73.1976.
Abstract
An in vitro comparison was made of the RNA polymerase activity associated with Newcastle disease virus (NDVo) and three clones of the temperature-sensitive mutant (NDVpi) isolated from persistently infected L cells. Less polymerase activity was associated with the NDVpi clones. Also, compared to NDVo, an increase in incubation temperature from 32 to 37 or 42 C resulted in a marked decrease in polymerase activity for the temperature-sensitive mutants which coincided with their inability to replicate at 42 C.
摘要
对与新城疫病毒（NDVo）以及从持续感染的L细胞中分离出的温度敏感突变体的三个克隆（NDVpi）相关的RNA聚合酶活性进行了体外比较。NDVpi克隆的聚合酶活性较低。此外，与NDVo相比，将孵育温度从32℃提高到37℃或42℃会导致温度敏感突变体的聚合酶活性显著降低，这与它们在42℃下无法复制相一致。
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