Wibawa Hendra, Noviana Deni, Tsuchiya Kimiyuki, Satruja Fadjar, Horii Yoichiro
Veterinary Teaching Hospital and Internal Medicine, Faculty of Agriculture, Miyazaki University, Gakuen-Kibanadai, Japan.
J Vet Med Sci. 2002 May;64(5):423-6. doi: 10.1292/jvms.64.423.
The resistance of cotton rats, Sigmodon hispidus to Nippostrongylus brasiliensis infection was examined and compared the response to that of the susceptible Indian soft-furred rat, Millardia meltada. After a primary infection with infective third-stage N. brasiliensis larvae (L3), the number of eggs in feces and adult worm recovery rates from the small intestine of cotton rats were significantly lower than in the controls. To determine whether cotton rat resistance was observed during the migratory phase or the intestinal phase, cotton rats and control animals were challenged subcutaneously with L3 or intraduodenally with adult worms, and larval recovery from lungs and adult worm burden were evaluated. The recovery rate of larvae from the lungs of cotton rats was about five-fold lower than from controls. Adult worm recovery from the small intestine of cotton rats was also lower than that from the controls, but the difference (two-fold lower) was smaller than that observed for lung recovery. Carbon treatment at a dose of 250-500 mg/kg effectively increased larval worm recovery from the lungs of cotton rats. However, this treatment had no effect on worm recovery from the intestine after intraduodenal implantation of adult N. brasiliensis. These results suggest that macrophage function have important role in the expression of strong resistance during the migratory phase of N. brasiliensis infection in cotton rats.
对棉鼠（Sigmodon hispidus）抵抗巴西日圆线虫感染的情况进行了研究，并将其反应与易感的印度软毛鼠（Millardia meltada）的反应进行了比较。在用感染性第三期巴西日圆线虫幼虫（L3）进行初次感染后，棉鼠粪便中的虫卵数量以及从小肠中回收的成虫回收率均显著低于对照组。为了确定棉鼠的抗性是在迁移阶段还是肠道阶段观察到的，对棉鼠和对照动物分别进行皮下注射L3或十二指肠内注射成虫的攻击，并评估从肺部回收的幼虫和成虫负荷。棉鼠肺部幼虫的回收率比对照组低约五倍。棉鼠小肠中回收的成虫也低于对照组，但差异（低两倍）小于肺部回收的差异。以250 - 500 mg/kg的剂量进行碳处理可有效提高棉鼠肺部幼虫的回收率。然而，这种处理对十二指肠内植入巴西日圆线虫成虫后从肠道回收的蠕虫没有影响。这些结果表明，巨噬细胞功能在棉鼠感染巴西日圆线虫的迁移阶段对强大抗性的表达具有重要作用。
