通过巢式聚合酶链反应从泰国断奶后多系统消耗综合征（PMWS）猪的福尔马林固定组织中检测猪圆环病毒2型（PCV - 2）DNA 。

Detection of porcine circovirus 2 (PCV-2) DNA by nested PCR from formalin-fixed tissues of post-weaning multisystemic wasting syndrome (PMWS) pigs in Thailand.

作者信息

Kiatipattanasakul-Banlunara Wijit, Tantilertcharoen Rachod, Suzuki Kazuhiko, Albarenque Stella Maris, Thanawongnuwech Roongroje, Nakayama Hiroyuki, Doi Kunio

机构信息

Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.

出版信息

J Vet Med Sci. 2002 May;64(5):449-52. doi: 10.1292/jvms.64.449.

DOI:10.1292/jvms.64.449

PMID:12069080

Abstract

A retrospective study for the detection of porcine circovirus 2 (PCV-2) DNA was conducted by nested PCR method in 16 cases of swine post-weaning multisystemic wasting syndrome (PMWS) in Thailand. Histopathology showed characteristic lesions of PMWS and intracytoplasmic viral inclusion bodies in macrophages infiltrating in lymphoid tissues. PCV-2 DNA was detected from formalin-fixed and/or formalin-fixed paraffin-embedded tissues from all pigs with PMWS. The amplified products were digested with Hae III.

摘要

采用巢式聚合酶链反应（PCR）方法，对泰国16例断奶后多系统消耗综合征（PMWS）猪病例进行了猪圆环病毒2型（PCV-2）DNA检测的回顾性研究。组织病理学显示了PMWS的特征性病变以及浸润于淋巴组织中的巨噬细胞内的胞浆内病毒包涵体。从所有患有PMWS的猪的福尔马林固定和/或福尔马林固定石蜡包埋组织中检测到了PCV-2 DNA。扩增产物用Hae III进行消化。