Ferris Rachel, Long L, Bunn S M, Robinson K M, Bradshaw H D, Rae A M, Taylor Gail
School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton S016 7PX, UK.
Tree Physiol. 2002 Jun;22(9):633-40. doi: 10.1093/treephys/22.9.633.
Genetic variation in stomatal initiation and density, and epidermal cell size and number were examined in a hybrid pedigree of Populus trichocarpa T. & G. and P. deltoides Marsh in both ambient ([aCO2]) and elevated ([eCO2]) concentrations of CO2. We aimed to link anatomical traits with the underlying genetic map of F2 Family 331, composed of 350 markers across 19 linkage groups. Leaf stomatal and epidermal cell traits showed pronounced differences between the original parents. We considered the following traits in the F2 population: stomatal density (SD), stomatal index (SI), epidermal cell area (ECA) and the number of epidermal cells per leaf (ECN). In [eCO2], adaxial SD and SI were reduced in the F2 population, whereas ECA increased and ECN remained unchanged. In [aCO2], four putative quantitative trait loci (QTL) with logarithm of the odds ratio (LOD) scores greater than 2.9 were found for stomatal traits on linkage group B: adaxial SI (LOD scores of 5.4 and 5.2); abaxial SI (LOD score of 3.3); and SD (LOD score of 3.2). These results imply that QTL for SI and SD share linkage group B and are under genetic control. More moderate LOD scores (LOD scores >/= 2.5) suggest QTL for SI on linkage groups A and B and for SD on linkage groups B, D and X with a probable co-locating quantitative trait locus for SI and SD on linkage group D (position 46.3 cM). The QTL in both [aCO2] and [eCO2] for adaxial SD were co-located on linkage group X (LOD scores of 3.5 and 2.6, respectively) indicating a similar response across both treatments. Putative QTL were located on linkage group A (position 89.2 cM) for both leaf size and ECN in [aCO2] and for ECA at almost the same position. The data provide preliminary evidence that leaf stomatal and cell traits are amenable to QTL analysis.
在二氧化碳环境浓度（[aCO2]）和升高浓度（[eCO2]）条件下，对毛果杨（Populus trichocarpa T. & G.）和三角叶杨（P. deltoides Marsh）的一个杂交谱系中的气孔起始和密度以及表皮细胞大小和数量的遗传变异进行了研究。我们旨在将解剖学性状与F2家系331的潜在遗传图谱联系起来，该家系由跨越19个连锁群的350个标记组成。叶片气孔和表皮细胞性状在原始亲本之间表现出明显差异。我们在F2群体中考虑了以下性状：气孔密度（SD）、气孔指数（SI）、表皮细胞面积（ECA）和每片叶的表皮细胞数量（ECN）。在[eCO2]条件下，F2群体中近轴面的SD和SI降低，而ECA增加且ECN保持不变。在[aCO2]条件下，在连锁群B上发现了四个对数优势比（LOD）得分大于2.9的气孔性状的假定数量性状位点（QTL）：近轴面SI（LOD得分分别为5.4和5.2）；远轴面SI（LOD得分3.3）；以及SD（LOD得分3.2）。这些结果表明，SI和SD的QTL共享连锁群B且受遗传控制。更中等的LOD得分（LOD得分≥2.5）表明连锁群A和B上存在SI的QTL，连锁群B、D和X上存在SD的QTL，并且连锁群D上可能存在SI和SD的共定位数量性状位点（位置46.3 cM）。在[aCO2]和[eCO2]条件下，近轴面SD的QTL共定位在连锁群X上（LOD得分分别为3.5和2.6），表明两种处理下有相似的响应。在[aCO2]条件下，叶片大小和ECN以及几乎相同位置的ECA的假定QTL位于连锁群A上（位置89.2 cM）。这些数据提供了初步证据，表明叶片气孔和细胞性状适合进行QTL分析。
