采用膜联蛋白A5成像方案在体内可视化细胞死亡。

Visualization of cell death in vivo with the annexin A5 imaging protocol.

作者信息

Reutelingsperger Chris P M, Dumont Ewald, Thimister Paul W, van Genderen Hugo, Kenis Heidi, van de Eijnde Stefan, Heidendal Guido, Hofstra Leo

机构信息

Department of Biochemistry of the Cardiovascular Research Institute Maastricht, University of Maastricht, P.O. Box 616, The Netherlands.

出版信息

J Immunol Methods. 2002 Jul 1;265(1-2):123-32. doi: 10.1016/s0022-1759(02)00075-3.

DOI:10.1016/s0022-1759(02)00075-3

PMID:12072183

Abstract

Annexin A5 binds to phosphatidylserine (PS), which is one of the "eat me" signals at the surface of the apoptotic cell. This property has been the driving force for the research of annexin A5 as a probe to measure apoptosis in vitro and in vivo. A non-invasive imaging protocol using annexin A5 has been developed and applied successfully to measure programmed cell death programmed cell death (PCD) in patients. This review highlights the aspects of this development and discusses clinical relevance, limitations and future perspectives of this approach of visualizing cell death.

摘要

膜联蛋白A5与磷脂酰丝氨酸（PS）结合，磷脂酰丝氨酸是凋亡细胞表面的“吃掉我”信号之一。这一特性一直是将膜联蛋白A5作为体外和体内测量细胞凋亡探针进行研究的驱动力。一种使用膜联蛋白A5的非侵入性成像方案已经开发出来，并成功应用于测量患者的程序性细胞死亡（PCD）。本综述重点介绍了这一进展的各个方面，并讨论了这种可视化细胞死亡方法的临床相关性、局限性和未来前景。