Corrochano L M
Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Avenida Reina Mercedes 6, Apartado 1095, E-41080 Seville, Spain.
Mol Genet Genomics. 2002 May;267(3):424-8. doi: 10.1007/s00438-002-0680-7. Epub 2002 May 9.
The zygomycete fungus Phycomyces blakesleeanus develops two types of fruiting bodies of very different size, macrophores and microphores. Blue light stimulates macrophorogenesis and inhibits microphorogenesis. I have adapted a method based on the polymerase chain reaction with arbitrary primers to investigate the role of differential gene expression during photophorogenesis in Phycomyces. Several cDNAs for genes induced in vegetative mycelium have been observed, but only one gene induced by blue light has been detected. I have demonstrated the feasibility of this approach by the isolation of a cDNA segment for the heat-shock protein HSP100 that is induced by blue light at the onset of sporangiophore development. The heat-shock protein HSP100 is an ATP-binding protein that has the ability to disassemble protein complexes. In plants, the gene for HSP100 is induced by light. The cDNA segment for HSP100 obtained from Phycomyces is 686 bp long and the predicted amino acid sequence contains one of the ATP-binding sites.
接合菌纲真菌布氏毛霉能形成两种大小差异极大的子实体，即大孢子囊和小孢子囊。蓝光刺激大孢子囊形成，抑制小孢子囊形成。我采用了一种基于任意引物聚合酶链反应的方法，来研究布氏毛霉在光诱导产孢过程中差异基因表达的作用。已观察到几个在营养菌丝体中诱导表达的基因的cDNA，但仅检测到一个受蓝光诱导的基因。我通过分离热休克蛋白HSP100的cDNA片段，证明了该方法的可行性，该片段在孢子囊梗发育开始时受蓝光诱导。热休克蛋白HSP100是一种具有ATP结合能力的蛋白质，能够拆解蛋白质复合物。在植物中，HSP100基因受光诱导。从布氏毛霉获得的HSP100的cDNA片段长686 bp，预测的氨基酸序列包含一个ATP结合位点。
