由葡萄球菌超抗原上调的牛CD26的分子特征

Molecular characterization of bovine CD26 upregulated by a staphylococcal superantigen.

作者信息

Lee Sang-Un, Park Yong-Ho, Davis William C, Hamilton Jo, Naessens Jan, Bohach Gregory A

机构信息

Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID 83844, USA.

出版信息

Immunogenetics. 2002 Jun;54(3):216-20. doi: 10.1007/s00251-002-0456-6. Epub 2002 Apr 20.

DOI:10.1007/s00251-002-0456-6

PMID:12073152

Abstract

In this report, we describe the cloning, sequencing, and expression of the bovine orthologue of CD26 (BoCD26). Several monoclonal antibodies specific for a molecule, activation molecule 3 (ACT3), aberrantly expressed on superantigen-stimulated bovine CD8(+) lymphocytes, reacted with recombinant BoCD26 expressed in COS-7 and CHO cells. We also showed that human CD8(+) T cells stimulated by a superantigen expressed CD26 at high levels. These results demonstrate that ACT3 is identical to BoCD26 and suggest that CD26 upregulation on CD8(+) T cells is a general phenomenon of superantigens and not limited to their effects on bovine cells.

摘要

在本报告中，我们描述了牛源CD26（BoCD26）直系同源物的克隆、测序及表达情况。几种针对超抗原刺激的牛CD8(+)淋巴细胞上异常表达的一种分子——激活分子3（ACT3）的单克隆抗体，能与在COS-7细胞和CHO细胞中表达的重组BoCD26发生反应。我们还发现，超抗原刺激的人CD8(+) T细胞会高水平表达CD26。这些结果表明，ACT3与BoCD26相同，并提示CD8(+) T细胞上CD26的上调是超抗原的普遍现象，并非仅限于其对牛细胞的作用。