Effects of D-glyceraldehyde and 3-o-methylglucose upon fluorescence of reduced pyridine nucleotides from perifused isolated pancreatic islets.

In perfused pancreatic islets, the fluorescence of reduced pyridine nucleotides was recorded continuously, D-Glyceraldehyde (5 mM) or 3-o-methylglucose (27.5 mM) never caused a net fluorescence increase. Since stepwise changes of the D-glucose concentration between 0 and 20 mM always induced a fluorescence increase, it is concluded that glucose on the one hand and glyceraldehyde or 3-o-methylglucose on the other hand cause different metabolic states in pancreatic islets.