[RP-HPLC determination of adenosine in fermented Cordyceps].

OBJECTIVE

A method for determination of adenosine in four fermented Cordyceps products by RP-HPLC is described.

METHOD

C18 column was used with methanol-0.06 mol/L potassium phosphate monobasic-tetrahydrofuran (10:150:1.5) as mobile phase. The flow rate was 1 ml/min and detected at 260 nm.

RESULTS

The average recovery was 98.7% and relative standard deviation was 1.10% (n = 5).

CONCLUSION

This method is simple, rapid and sensitive. The paper introduces the feasibility of supersonic extraction method taking the place of reflux method.