[反相高效液相色谱法同时测定天麻中四种成分]

[Simultaneous determination of four components in Gastrodia elata by RP-HPLC].

作者信息

Yan Baoqing, Zhang Huifen, Pang Nannan, Chen Xiaohui, Yu Zhiguo, Bi Kaishun

机构信息

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(22):2903-6.

PMID:20209956

Abstract

OBJECTIVE

To develop a reversed-phase HPLC method for simultaneous determination of gastrodin, adenosin, 4-hydroxybenzyl alcohol and 4-hydroxybenzaldehyde in Rhizoma Gastrodia.

METHOD

A Kromasil C18 column (4.6 mm x 250 mm, 5 microm) was used with a methanol-water-0.1% acetic acid gradient elution system. The eluates were detected at 270 nm, the flow rate was 1.0 mL x min(-1) and the column temperature was 35 degrees C.

RESULT

The linear range of gastrodin, adenosin, 4-hydroxybenzyl alcohol and 4-hydroxybenzaldehyde were 19.1-383 (r = 0.999 9), 0.620-12.4 (r = 0.999 9), 2.45-49.0 (r = 0.999 9), 0.280-5.63 mg x L(-1) (r = 0.999 6), respectively. The average recoveries (n = 9) of the four components were 96.7% -97.7%, RSD < 1.6%.

CONCLUSION

The method is accurate, sensitive and reliable for determination of gastrodin, adenosin, 4-hydroxybenzyl alcohol and 4-hydroxybenzaldehyde in Rhizoma Gastrodia.

摘要

目的

建立一种反相高效液相色谱法同时测定天麻中天麻素、腺苷、4-羟基苄醇和4-羟基苯甲醛的含量。

方法

采用Kromasil C18柱（4.6 mm×250 mm，5μm），以甲醇-水-0.1%乙酸梯度洗脱体系进行洗脱。在270 nm波长处检测洗脱液，流速为1.0 mL·min⁻¹，柱温为35℃。

结果

天麻素、腺苷、4-羟基苄醇和4-羟基苯甲醛的线性范围分别为19.1 - 383（r = 0.999 9）、0.620 - 12.4（r = 0.999 9）、2.45 - 49.0（r = 0.999 9）、0.280 - 5.63 mg·L⁻¹（r = 0.999 6）。4种成分的平均回收率（n = 9）为96.7% - 97.7%，相对标准偏差（RSD）< 1.6%。