Hofacker Ivo L, Fekete Martin, Stadler Peter F
Institut für Theoretische Chemie, Universität Wien, Währingerstrasse 17, Austria.
J Mol Biol. 2002 Jun 21;319(5):1059-66. doi: 10.1016/S0022-2836(02)00308-X.
Most functional RNA molecules have characteristic secondary structures that are highly conserved in evolution. Here we present a method for computing the consensus structure of a set aligned RNA sequences taking into account both thermodynamic stability and sequence covariation. Comparison with phylogenetic structures of rRNAs shows that a reliability of prediction of more than 80% is achieved for only five related sequences. As an application we show that the Early Noduline mRNA contains significant secondary structure that is supported by sequence covariation.
大多数功能性RNA分子具有在进化中高度保守的特征性二级结构。在此，我们提出一种计算一组比对RNA序列的共有结构的方法，该方法同时考虑了热力学稳定性和序列共变。与rRNA的系统发育结构比较表明，仅对于五个相关序列，预测可靠性就超过了80%。作为一个应用实例，我们表明早期结瘤素mRNA含有由序列共变支持的显著二级结构。
