Wolfer David P, Crusio Wim E, Lipp Hans Peter
Institute of Anatomy and Center for Neuroscience, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
Trends Neurosci. 2002 Jul;25(7):336-40. doi: 10.1016/s0166-2236(02)02192-6.
Inducing null mutations by means of homologous recombination provides a powerful technique to investigate gene function and has found wide application in many different fields. However, it was realized some time ago that the specific way in which such knockout mutants are generated can be confounding, making it impossible to separate the effects of the induced null mutation from those of alleles originating from the embryonic stem cell donor. In addition, effects from null mutations can be altered on different genetic backgrounds. Here we present some simple breeding strategies to test for flanking gene effects that are compatible with the recommendations of the Banbury Conference on Genetic Background in Mice and with common practices of creating and maintaining mouse knockout lines.
通过同源重组诱导无效突变提供了一种研究基因功能的强大技术，并已在许多不同领域得到广泛应用。然而，人们在一段时间前就意识到，产生此类基因敲除突变体的特定方式可能会造成混淆，使得无法将诱导的无效突变的影响与源自胚胎干细胞供体的等位基因的影响区分开来。此外，无效突变的影响在不同的遗传背景下可能会发生改变。在此，我们提出一些简单的育种策略，以测试侧翼基因效应，这些策略符合关于小鼠遗传背景的班伯里会议的建议以及创建和维持小鼠基因敲除品系的常规做法。
