Schmitt T, Seitz A
Institut für Zoologie, Abt. V Okologie, Saarstrasse 21, 55099 Mainz, Germany.
Heredity (Edinb). 2002 Jul;89(1):20-6. doi: 10.1038/sj.hdy.6800087.
The genetic population structure of Polyommatus coridon (Poda, 1761) (Chalk-hill blue) was studied by means of allozyme electrophoresis in north-eastern Germany, the Czech Republic, Slovakia and Hungary. All analysed parameters showed high genetic diversity within populations (number of alleles: 2.61; observed and expected heterozygosity: 18.6% and 19.7%, respectively; percentage of polymorphic loci: total: 73.6%, on 95% level: 56.1%), whereas genetic differentiation between populations was comparatively low (F(ST) = 0.028 +/- 0.005 s.d.). Hierarchical variance analysis revealed significant structuring among five regional population clusters. A significant isolation-by-distance structure exists (r = 0.39; P < 0.05). The mean number of alleles per locus declined significantly from south to north and showed a strong correlation with the geographical latitude (r = -0.88, P < 0.0001). We suggest that this reflects the loss of alleles during the postglacial colonisation of eastern Central Europe from an ice-age refugium in the Balkans. A possible scenario for the postglacial expansion process in eastern Central Europe is discussed using these data: coming from the north-western part of the Balkans, P. coridon may have reached the western tip of Hungary, and consecutively colonised eastern Central Europe using two alternative expansion routes.
通过等位酶电泳技术，对德国东北部、捷克共和国、斯洛伐克和匈牙利的蓝灰蝶（Polyommatus coridon (Poda, 1761)）的遗传种群结构进行了研究。所有分析参数均显示种群内具有高度的遗传多样性（等位基因数：2.61；观察到的杂合度和期望杂合度分别为18.6%和19.7%；多态位点百分比：总计73.6%，在95%水平上为56.1%），而种群间的遗传分化相对较低（F(ST) = 0.028 ± 0.005标准差）。层次方差分析揭示了五个区域种群集群之间存在显著的结构。存在显著的距离隔离结构（r = 0.39；P < 0.05）。每个位点的等位基因平均数从南向北显著下降，并与地理纬度呈现出强烈的相关性（r = -0.88，P < 0.0001）。我们认为，这反映了在末次冰期后从中欧东部由巴尔干半岛的冰期避难所进行殖民化过程中等位基因的丢失。利用这些数据讨论了中欧东部末次冰期后扩张过程的一种可能情景：蓝灰蝶可能来自巴尔干半岛的西北部，到达匈牙利的西端，并随后通过两条替代扩张路线相继殖民化了中欧东部。
