软骨细胞对水凝胶的生物学反应。

Biological response of chondrocytes to hydrogels.

作者信息

Elisseeff J H, Lee A, Kleinman H K, Yamada Y

机构信息

Whitaker Institute of Biomedical Engineering, Department of Biomedical Engineering, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USA.

出版信息

Ann N Y Acad Sci. 2002 Jun;961:118-22. doi: 10.1111/j.1749-6632.2002.tb03062.x.

DOI:10.1111/j.1749-6632.2002.tb03062.x

PMID:12081878

Abstract

Primary bovine chondrocytes were encapsulated in alginate and alginate combined with cartilage matrix extract, Cartrigel, for the purpose of cartilage tissue engineering. The cell constructs were incubated in vitro and gene expression of cartilage-specific extracellular matrix molecules was quantitated and localized with in situ hybridization with a decrease in expression observed in the alginate-Cartrigel constructs. Further understanding of cell response to scaffolds will allow rational design and development of hydrogels for cartilage tissue engineering.

摘要

为了进行软骨组织工程，将原代牛软骨细胞封装在藻酸盐以及藻酸盐与软骨基质提取物（Cartrigel）的组合中。细胞构建体在体外培养，通过原位杂交对软骨特异性细胞外基质分子的基因表达进行定量和定位，结果发现在藻酸盐 - Cartrigel构建体中表达有所下降。对细胞对支架反应的进一步了解将有助于合理设计和开发用于软骨组织工程的水凝胶。