Romanova I B, Chervina L V
Prikl Biokhim Mikrobiol. 1975 Jul-Aug;11(4):608-12.
The method of potarographic assay of nicotinic acid in the culure liquid has been developed. The effect of all the constituents of the culture liquid on the accuracy of measurements has been tested. The analytic scheme which provides elimination of interfering components has been devised. As the polarographic medium 0.1 n HCl was used. The quantity of nicotinic acid was measured with respect to the wave of E1/2 = - 1.18 v. The described method makes it possible to identify nicotinic acid at concentrations of 3.3 X 10(-4) M to 3.3 X 10(-3) M in the culture liquid with an error of not more than +/-5%.
已开发出测定培养液中烟酸的极谱分析方法。测试了培养液中所有成分对测量准确性的影响。设计了可消除干扰成分的分析方案。使用0.1N�0.1 n HCl作为极谱介质。根据E1/2 = - 1.18 v的波测定烟酸的量。所述方法能够在培养液中识别浓度为3.3×10(-4) M至3.3×10(-3) M的烟酸,误差不超过±5%。