Barros Marilia, De Souza Silva M A, Huston Joseph P, Tomaz Carlos
Laboratory of Neurobiology, Primate Center and Department of Physiological Sciences, Institute of Biology, University of Brasilia, C.P. 04631, DF, Brasília, Brazil.
Peptides. 2002 May;23(5):967-73. doi: 10.1016/s0196-9781(02)00020-7.
The behavioral effects of the amino (N)-terminal fragment of substance P (SP(1-7)) on the marmoset (Callithrix penicillata) predator confrontation test of fear/anxiety were investigated. The test apparatus consisted of a figure-eight maze with three parallel arms interconnected at each extremity to a perpendicular arm. A taxidermized oncilla cat (Felis tigrina) was placed outside the maze facing one of its corners. Subjects were submitted to seven 30 min maze habituation trials (HTs), in the absence of the 'predator', and then to six 30 min treatment trials (TTs), in the presence of the 'predator', consisting of four doses of SP(1-7) (5, 50, 250 and 500 microg/kg; IP), saline and sham injection. SP(1-7) treatment reversed, in a dose-dependent way, the fear-induced avoidance behavior due to the predator's presence and increased the frequency of exploratory behaviors. Locomotor activity decreased during successive HTs, yet increased after all SP(1-7) treatments. These results indicate that systemic administration of SP(1-7) produces anxiolytic-like effects in marmosets tested in the predator confrontation model of fear/anxiety.
研究了P物质（SP(1-7)）的氨基（N）末端片段对狨猴（Callithrix penicillata）恐惧/焦虑捕食者对抗试验的行为影响。测试装置由一个数字八迷宫组成，有三个平行臂，在每个末端与一个垂直臂相连。一只填充的虎猫（Felis tigrina）放在迷宫外面，面向其中一个角落。在没有“捕食者”的情况下，让受试者进行七次30分钟的迷宫习惯化试验（HTs），然后在有“捕食者”的情况下进行六次30分钟的治疗试验（TTs），包括四种剂量的SP(1-7)（5、50、250和500微克/千克；腹腔注射）、生理盐水和假注射。SP(1-7)治疗以剂量依赖的方式逆转了由于捕食者的存在而引起的恐惧诱导回避行为，并增加了探索行为的频率。在连续的HTs期间运动活动减少，但在所有SP(1-7)治疗后增加。这些结果表明，在恐惧/焦虑捕食者对抗模型中测试的狨猴中，全身性给予SP(1-7)会产生抗焦虑样作用。
