Ryan W L, Short N A, Curtis G L
Proc Soc Exp Biol Med. 1975 Dec;150(3):699-702. doi: 10.3181/00379727-150-39109.
Adenylate cyclase activity of a rat embryo fibroblast cell line (F111) is markedly increased by brief treatment with 1:300 trypsin. The degree of stimulation depends upon the length of time the cells are treated and the concentration of trypsin. Crystalline trypsin produced a stimulation similar to that obtained with 1:300 trypsin. Further, the addition of soybean trypsin inhibitor blocked the stimulation of adenylate cyclase by 1:300 trypsin. Trypsin-treated adenylate cyclase responds to PGE1, but there is no increase over that of untreated enzyme. This result and the increase in fluoride-stimulated levels of activity suggest that the trypsin is acting upon the catalytic unit of the enzyme.
用1:300的胰蛋白酶短暂处理大鼠胚胎成纤维细胞系（F111），其腺苷酸环化酶活性显著增加。刺激程度取决于细胞处理的时间长度和胰蛋白酶的浓度。结晶胰蛋白酶产生的刺激作用与1:300的胰蛋白酶相似。此外，添加大豆胰蛋白酶抑制剂可阻断1:300胰蛋白酶对腺苷酸环化酶的刺激作用。经胰蛋白酶处理的腺苷酸环化酶对前列腺素E1有反应，但与未处理的酶相比没有增加。这一结果以及氟化物刺激活性水平的增加表明，胰蛋白酶作用于该酶的催化单位。
