健康受试者和生长激素缺乏患者运动期间的血浆胃饥饿素水平。

Plasma ghrelin levels during exercise in healthy subjects and in growth hormone-deficient patients.

作者信息

Dall Rolf, Kanaley Jill, Hansen Troels Krarup, Møller Niels, Christiansen Jens S, Hosoda Hiroshi, Kangawa Kenji, Jørgensen Jens O L

机构信息

Medical Department M (Endocrinology and Diabetes) and Institute of Experimental Clinical Research, Aarhus University Hospital, Aarhus, Denmark.

出版信息

Eur J Endocrinol. 2002 Jul;147(1):65-70. doi: 10.1530/eje.0.1470065.

DOI:10.1530/eje.0.1470065

PMID:12088921

Abstract

OBJECTIVE

To characterise plasma levels of the recently identified endogenous ligand for the GH secretagogue receptor (ghrelin) during submaximal aerobic exercise in healthy adults and in GH-deficient adults.

DESIGN

Eight healthy males (mean+/-s.e. age, 40.8+/-2.9 years) and eight hypopituitary males with verified GH deficiency (mean+/-s.e. age, 40.8+/-4.7 years) underwent a baseline test of their peak aerobic capacity (VO(2) peak) and lactate threshold (LT) on a cycle ergometer, as well as an evaluation of body composition. The patients were then studied on two occasions in random order when they exercised for 45 min at their LT. On one occasion, GH replacement had been discontinued from the evening before, whereas on the other occasion they received their evening GH in addition to an intravenous infusion of GH (0.4 IU) during exercise the following day. The healthy subjects exercised at their LT on one occasion without GH.

RESULTS

The patients were significantly more obese and had lower VO(2) max (corrected for body weight) and LT as compared with the control subjects. Exercise induced a peak in serum GH concentrations after 45 min in the control group (11.43+/-3.61 microg/l). Infusion of GH in the patients resulted in a peak level after 45 min, whereas no increase was detected when exercising without GH (9.77+/-2.40 (GH) vs 0.11+/-0.07 microg/l (no GH)). Plasma ghrelin levels did not change significantly with time in either study, and no correlations were detected between ghrelin levels and parameters such as GH and IGF-I levels, age or body composition. Plasma ghrelin levels were significantly lower during the study period with GH as compared with the study with no GH.

CONCLUSIONS

Submaximal aerobic exercise of an intensity sufficient to stimulate GH release was not associated with significant alterations in plasma ghrelin concentrations, which indicated that systemic ghrelin is not involved in the exercise-induced stimulation of GH secretion. The observation that ghrelin levels were lower during GH replacement suggests that GH may feedback-inhibit systemic ghrelin release.

摘要

目的

测定健康成年人及生长激素缺乏成年人在次最大强度有氧运动期间，生长激素促分泌素受体（ghrelin）的新近鉴定的内源性配体的血浆水平。

设计

8名健康男性（平均±标准误年龄，40.8±2.9岁）和8名经证实生长激素缺乏的垂体功能减退男性（平均±标准误年龄，40.8±4.7岁）在自行车测力计上进行了峰值有氧运动能力（VO₂峰值）和乳酸阈值（LT）的基线测试，以及身体成分评估。然后，患者以随机顺序在两次研究中进行，他们在乳酸阈值下运动45分钟。一次，从运动前一晚开始停用生长激素替代治疗，而在另一次研究中，他们在运动前一晚接受生长激素替代治疗，并在第二天运动期间静脉输注生长激素（0.4IU）。健康受试者在一次研究中在乳酸阈值下运动但未使用生长激素。

结果

与对照组相比，患者明显更肥胖，且最大摄氧量（校正体重后）和乳酸阈值更低。运动45分钟后，对照组血清生长激素浓度达到峰值（11.43±3.61μg/L）。患者输注生长激素后45分钟达到峰值水平，而未使用生长激素运动时未检测到生长激素增加（使用生长激素时为9.77±2.40（μg/L），未使用生长激素时为0.11±0.07μg/L）。在两项研究中，血浆ghrelin水平均未随时间发生显著变化，且未检测到ghrelin水平与生长激素、胰岛素样生长因子-I水平、年龄或身体成分等参数之间的相关性。与未使用生长激素的研究相比，使用生长激素的研究期间血浆ghrelin水平显著更低。