Weise J B, Goeroegh T, Meyer J E, Maune S
Department of Otorhinolaryngology, Head and Neck Surgery, Christian-Albrechts-University, Kiel, Germany.
Res Commun Mol Pathol Pharmacol. 2001 Jul-Aug;110(1-2):139-42.
Chemokines in biological sample are frequently found at very low level. Further, concentrations of chemokines which were measured in the same tissue detected with different methods often differ in literature. Therefore, RANTES concentrations were quantified by application of sandwich enzyme-linked immunosorbent assay (ELISA) and Western blot analyses. The sensitivity of ELISA was found to be much higher than that of Western blot. Additionally, the detection time differed considerably as well. For biological and biochemical characterization of chemokines it is essential to implement the optimal purification and detection techniques. With an understanding of the technical procedures and some pitfalls, chemokine detection can be applied more reliably.
生物样本中的趋化因子浓度常常非常低。此外，在文献中，用不同方法检测同一组织中测得的趋化因子浓度往往存在差异。因此，通过夹心酶联免疫吸附测定（ELISA）和蛋白质免疫印迹分析对RANTES浓度进行了定量。发现ELISA的灵敏度远高于蛋白质免疫印迹法。此外，检测时间也有很大差异。对于趋化因子的生物学和生物化学特性鉴定，采用最佳的纯化和检测技术至关重要。了解技术流程和一些注意事项后，趋化因子检测可以更可靠地进行。
