Kozar Rosemary A, Hu Sen, Hassoun Heitham T, DeSoignie Roland, Moore Frederick A
Department of Surgery, University of Texas-Houston, Health Science Center, 77030, USA.
JPEN J Parenter Enteral Nutr. 2002 Jul-Aug;26(4):226-9. doi: 10.1177/0148607102026004226.
We have previously demonstrated in a rodent model the differential effects that specific intraluminal nutrients exert on gut ischemia/reperfusion (I/R) injury. Alanine was shown to amplify, whereas glucose protected against, gut I/R injury and associated gut dysfunction. The objective of this study was to determine whether these specific nutrients are associated with alterations in mucosal perfusion during gut I/R.
Sprague-Dawley rats had either a laser doppler probe or a tonometer inserted into a jejunal sac filled with either 10 mmol/L alanine, glucose, or mannitol (osmotic control) followed by 60 minutes of superior mesenteric artery occlusion and 60 minutes of reperfusion. Laser doppler mucosal blood flow and regional PCO2 (PrCO2) measurements were obtained.
Mucosal blood flow was significantly increased during both ischemia and reperfusion when intraluminal glucose was present compared with intraluminal alanine. Blood flow changes were reflected by lower jejunal PrCO2 measurements with intraluminal glucose compared with intraluminal alanine.
Intraluminal glucose can augment mucosal blood flow during gut I/R and may explain the protective effect we previously observed.
我们之前在啮齿动物模型中证明了特定腔内营养素对肠道缺血/再灌注（I/R）损伤的不同影响。结果显示，丙氨酸会加剧肠道I/R损伤，而葡萄糖则可预防该损伤及相关的肠道功能障碍。本研究的目的是确定这些特定营养素是否与肠道I/R期间黏膜灌注的改变有关。
将激光多普勒探头或眼压计插入充满10 mmol/L丙氨酸、葡萄糖或甘露醇（渗透对照）的空肠囊中，随后对Sprague-Dawley大鼠进行60分钟的肠系膜上动脉闭塞和60分钟的再灌注。测量激光多普勒黏膜血流量和局部PCO2（PrCO2）。
与腔内丙氨酸相比，腔内存在葡萄糖时，缺血和再灌注期间黏膜血流量均显著增加。与腔内丙氨酸相比，腔内葡萄糖组空肠PrCO2测量值较低，这反映了血流变化。
腔内葡萄糖可增加肠道I/R期间的黏膜血流量，这可能解释了我们之前观察到的保护作用。
