Barrett Gordon M, Shimizu Keiko, Bardi Massimo, Mori Akio
Primate Research Institute, Kyoto University, Kanrin, Inuyama, Aichi 484-8506, Japan.
Primates. 2002 Jan;43(1):29-39. doi: 10.1007/BF02629574.
Validation of a simple method for the extraction and quantification of testosterone (T) from the excreta of male Japanese macaques (Macaca fuscata) is presented. Radioimmunoassay of paired fecal and serum samples collected from four intact sexually mature males during the breeding season provided profiles that were significantly correlated when samples were offset by approximately 48 hr. Additionally, no significant differences were observed in the pattern of temporal variation of T levels in serum and feces. Two castrated males were injected with radioinert T, and the patterns of excretion were observed by analysis of serial fecal and urine samples. Approximately 48 hr after the steroid was administered, a significant peak in the average fecal T levels was apparent. The injection event was also registered in the urine of both males, although qualitative differences were observed. These data suggest that measures of fecal T provide a reliable and non-invasive means of assessing gonadal function in this species. As the analysis of hormone levels in feces allows for frequent, stress-free sampling with minimal disruption, this method should be preferred in long-term or in situ applications requiring endocrine monitoring.
本文介绍了一种从雄性日本猕猴（Macaca fuscata）排泄物中提取和定量睾酮（T）的简单方法的验证。在繁殖季节，对四只性成熟未阉割雄性猕猴采集的粪便和血清配对样本进行放射免疫分析，当样本偏移约48小时时，得到的曲线具有显著相关性。此外，血清和粪便中T水平的时间变化模式没有显著差异。对两只阉割雄性猕猴注射放射性惰性T，并通过分析连续的粪便和尿液样本观察排泄模式。给药后约48小时，粪便中T的平均水平出现显著峰值。两只猕猴的尿液中也记录到了注射事件，不过观察到了定性差异。这些数据表明，粪便T的测量为评估该物种的性腺功能提供了一种可靠且非侵入性的方法。由于对粪便中激素水平的分析允许进行频繁、无应激的采样，且干扰最小，因此在需要内分泌监测的长期或原位应用中，该方法应是首选。
