Lisby Gorm, Gutschik Ernö, Durack David T
Department of Clinical Microbiology, Hvidovre Hospital, University of Copenhagen, Kettegårds Alle 30, 2650 Hvidovre, Denmark.
Infect Dis Clin North Am. 2002 Jun;16(2):393-412, x. doi: 10.1016/s0891-5520(01)00002-2.
The culture of viable microorganisms from the blood or from cardiac tissue is currently the most important test for diagnosis of IE. This is followed by phenotypic identification methods used for taxonomic positioning of isolates. However, in those cases where the invading microorganism is difficult or impossible to culture (including instances of prior antimicrobial treatment), molecular methods provide the best means for detection. Molecular identification methods, either nucleic acid target or signal amplification alone or in combination with sequence analysis can offer a more specific and in some cases a more rapid alternative to the phenotypic methods. We propose revised Duke criteria of IE, including positive identification of an organism by molecular biology methods.
从血液或心脏组织中培养活的微生物目前是诊断感染性心内膜炎（IE）最重要的检测方法。其次是用于对分离株进行分类定位的表型鉴定方法。然而，在那些入侵微生物难以培养或无法培养的情况下（包括先前接受过抗菌治疗的情况），分子方法是最佳的检测手段。分子鉴定方法，无论是单独的核酸靶标或信号扩增，还是与序列分析相结合，都可以提供一种比表型方法更具特异性，且在某些情况下更快速的替代方法。我们提出了修订后的IE杜克标准，包括通过分子生物学方法对病原体进行阳性鉴定。
