一种用于吡哆醛的具有高特异性和灵敏度的酶促荧光测定法。
An enzymatic fluorometric assay for pyridoxal with high specificity and sensitivity.
作者信息
Trongpanich Yanee, Mito Mitsunori, Yagi Toshiharu
机构信息
Department of Bioresources Science, Faculty of Agriculture, Kochi University, Japan.
出版信息
Biosci Biotechnol Biochem. 2002 May;66(5):1152-4. doi: 10.1271/bbb.66.1152.
An enzymatic fluorometric assay for pyridoxal with pyridoxal dehydrogenase was developed. The detection limit was about 10 pmol: the calibration curve of pyridoxal showed high linearity (r=0.993). The values obtained by this method correlated well with those by the HPLC method. The enzyme had a high specificity for pyridoxal, and thus animal samples could be directly analyzed without separation of pyridoxal 5'-phosphate by column chromatography.
开发了一种用吡哆醛脱氢酶测定吡哆醛的酶荧光测定法。检测限约为10皮摩尔：吡哆醛的校准曲线显示出高线性（r = 0.993）。该方法获得的值与高效液相色谱法获得的值相关性良好。该酶对吡哆醛具有高度特异性，因此动物样品无需通过柱色谱分离5'-磷酸吡哆醛即可直接分析。
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