Nadir M T, Gilbert P
Microbios. 1979;26(103):51-63.
Chlorhexidine (0.5-0.65 microM) and Triton X45 (30-40 microM) added to exponential phase Bacillus megaterium KM- cultures was growth inhibitory. The presence of KCl (0.05-0.35 M) in the medium did not significantly affect growth rate in the absence of drug, yet reduced the growth inhibitory activity of the chlorhexidine and enhanced that of Triton X45. These effects were maximal at KCl concentrations of 0.2 M and above, when complete protection towards chlorhexidine and lysis of the cultures in the presence of Triton X45 were observed. Time-survivor curves in the presence of chlorhexidine (0.7-1.0 microM) gave LT90 values of 1.5-2.0 h in the absence of KCl, yet its inclusion (0.35 M) totally inhibited this low level bactericidal activity. Drug absorption by whole cell and isolated cell wall preparations was determined in the presence and absence of KCl (0.35 M). Chlorhexidine uptake by intact cells was reduced by approximately 50% in the presence of salt whereas that of Triton X45 increased by a similar fraction. Uptake of chlorhexidine by the cell wall fraction accounted for approximately 50% of that for the whole cells and was relatively unaffected by the presence of KCl. Conversely, absorption of Triton X45 by the cell wall fraction accounted for most of the uptake by whole cells and increased markedly in the presence of salts.
将洗必泰（0.5 - 0.65微摩尔）和吐温X45（30 - 40微摩尔）添加到处于指数生长期的巨大芽孢杆菌KM - 培养物中具有生长抑制作用。在无药物的情况下，培养基中KCl（0.05 - 0.35摩尔）的存在对生长速率没有显著影响，但降低了洗必泰的生长抑制活性并增强了吐温X45的生长抑制活性。当KCl浓度达到0.2摩尔及以上时，这些影响最为显著，此时观察到对洗必泰的完全保护以及在吐温X45存在下培养物的裂解。在存在洗必泰（0.7 - 1.0微摩尔）的情况下，无KCl时的时间 - 存活曲线给出的LT90值为1.5 - 2.0小时，但其加入（0.35摩尔）完全抑制了这种低水平的杀菌活性。在有和无KCl（0.35摩尔）的情况下测定了全细胞和分离的细胞壁制剂对药物的吸收。在有盐存在的情况下，完整细胞对洗必泰的摄取减少了约50%，而吐温X45的摄取增加了相似的比例。细胞壁部分对洗必泰的摄取约占全细胞摄取的50%，并且相对不受KCl存在的影响。相反，细胞壁部分对吐温X45的吸收占全细胞摄取的大部分，并且在有盐存在时显著增加。
