Ye X Y, Ng T B
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories.
J Pept Sci. 2002 Jun;8(6):235-40. doi: 10.1002/psc.382.
From the fresh fruiting bodies of the oyster mushroom a peptide with a molecular weight of 9 kDa and demonstrating a novel N-terminal sequence GPCYLVAFYESSGRR was isolated. The isolation procedure involved ion exchange chromatography on CM-Sepharose and Mono S. The peptide was adsorbed on both types of chromatographic media. The peptide demonstrated a ribonuclease activity of 650 U/mg toward yeast transfer RNA. It inhibited cell-free translation in a rabbit reticulocyte lysate system with an IC50 of 15 nM.
从平菇的新鲜子实体中分离出一种分子量为9 kDa的肽，其N端序列为GPCYLVAFYESSGRR，具有新颖性。分离过程包括在CM-琼脂糖凝胶和Mono S上进行离子交换色谱。该肽能吸附在这两种色谱介质上。该肽对酵母转移RNA的核糖核酸酶活性为650 U/mg。在兔网织红细胞裂解物系统中，它能抑制无细胞翻译，IC50为15 nM。
