Spiegel Ivo, Salomon Daniela, Erne Beat, Schaeren-Wiemers Nicole, Peles Elior
Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel.
Mol Cell Neurosci. 2002 Jun;20(2):283-97. doi: 10.1006/mcne.2002.1110.
The NCP family of cell-recognition molecules represents a distinct subgroup of the neurexins that includes Caspr and Caspr2, as well as Drosophila Neurexin-IV and axotactin. Here, we report the identification of Caspr3 and Caspr4, two new NCPs expressed in nervous system. Caspr3 was detected along axons in the corpus callosum, spinal cord, basket cells in the cerebellum and in peripheral nerves, as well as in oligodendrocytes. In contrast, expression of Caspr4 was more restricted to specific neuronal subpopulations in the olfactory bulb, hippocampus, deep cerebellar nuclei, and the substantia nigra. Similar to the neurexins, the cytoplasmic tails of Caspr3 and Caspr4 interacted differentially with PDZ domain-containing proteins of the CASK/Lin2-Veli/Lin7-Mint1/Lin10 complex. The structural organization and distinct cellular distribution of Caspr3 and Caspr4 suggest a potential role of these proteins in cell recognition within the nervous system.
细胞识别分子的NCP家族代表了神经配蛋白的一个独特亚组，其中包括接触蛋白相关蛋白（Caspr）和接触蛋白相关蛋白2（Caspr2），以及果蝇神经配蛋白-IV和轴突结合素。在此，我们报告了在神经系统中表达的两种新的NCP，即Caspr3和Caspr4的鉴定。在胼胝体、脊髓、小脑篮状细胞、外周神经以及少突胶质细胞的轴突上检测到了Caspr3。相比之下，Caspr4的表达更局限于嗅球、海马体、小脑深部核团和黑质中的特定神经元亚群。与神经配蛋白类似，Caspr3和Caspr4的胞质尾巴与CASK/Lin2-Veli/Lin7-Mint1/Lin10复合体中含PDZ结构域的蛋白质发生不同的相互作用。Caspr3和Caspr4的结构组织和独特的细胞分布表明这些蛋白质在神经系统的细胞识别中可能发挥作用。
