Fernández-González A, Badía R, Díaz-García M E
Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain.
J Pharm Biomed Anal. 2002 Jul 20;29(4):669-79. doi: 10.1016/s0731-7085(02)00185-1.
A study has been made on the spectrophotometric determination of the beta-lactamic antibiotics, amoxicillin and ampicillin, in micellar media using Cu ions as catalyst. Batch and flow injection approaches were compared. Multisimplex design was used to determine the optimal values of the flow injection analysis (FIA) system. Chemical (buffer, pH and Cu(II) concentrations) and physical (flow rate, temperature and reaction coil length) variables were considered. The analytical performance characteristics were as follows: the detection limits for batch and flow-through systems were 2.5 x 10(-7) and 2 x 10(-6) M, respectively, and a relative standard deviation less than 1% was found for both methods. The proposed FIA methodology was satisfactorily applied to the determination of the antibiotics in pharmaceutical formulations.
开展了一项关于使用铜离子作为催化剂，在胶束介质中分光光度法测定β-内酰胺类抗生素阿莫西林和氨苄西林的研究。比较了分批进样和流动注射方法。采用多单纯形设计来确定流动注射分析（FIA）系统的最佳值。考虑了化学变量（缓冲液、pH值和铜（II）浓度）和物理变量（流速、温度和反应盘管长度）。分析性能特征如下：分批进样系统和流通系统的检测限分别为2.5×10⁻⁷和2×10⁻⁶ M，两种方法的相对标准偏差均小于1%。所提出的FIA方法成功应用于药物制剂中抗生素的测定。
