Chong Parkson Lee-Gau, Sugár István P
Department of Biochemistry, Temple University School of Medicine, 3420 N. Broad St., Philadelphia, PA 19140, USA.
Chem Phys Lipids. 2002 Jun;116(1-2):153-75. doi: 10.1016/s0009-3084(02)00025-7.
This article reviews the use of fluorescent lipids and free probes in the studies of lipid regular distribution in model membranes. The first part of this article summarizes the evidence and physical properties for lipid regular distribution in pyrene-labeled phosphatidylcholine (PC)/unlabeled PC binary mixtures as revealed by the fluorescence of pyrene-labeled PC. The original and the extended hexagonal superlattice model are discussed. The second part focuses on the fluorescence studies of sterol regular distributions in membranes. The experimental evidence for sterol superlattice formation obtained from the fluorescent sterol (i.e. dehydroergosterol) and non-sterol fluorescent probes (e.g. DPH and Laurdan) are evaluated. Prospects and concerns are given with regard to the sterol regular distribution. The third part deals briefly with the evidence for polar headgroup superlattices. The emphasis of this article is placed on the new concept that membrane properties and activities, including the activities of surface acting enzymes, drug partitioning, and membrane free volume, are fine-tuned by minute changes in the concentration of bulky lipids (e.g. sterols and pyrene-containing acyl chains) in the vicinities of the critical mole fractions for superlattice formation.
本文综述了荧光脂质和游离探针在模型膜脂质规则分布研究中的应用。本文第一部分总结了芘标记的磷脂酰胆碱（PC）/未标记的PC二元混合物中脂质规则分布的证据和物理性质，这些是通过芘标记的PC的荧光揭示的。讨论了原始的和扩展的六方超晶格模型。第二部分重点关注膜中甾醇规则分布的荧光研究。评估了从荧光甾醇（即脱氢麦角甾醇）和非甾醇荧光探针（如DPH和Laurdan）获得的甾醇超晶格形成的实验证据。给出了关于甾醇规则分布的前景和关注点。第三部分简要论述了极性头部基团超晶格的证据。本文重点关注的新概念是，膜的性质和活性，包括表面活性酶的活性、药物分配和膜自由体积，通过在超晶格形成的临界摩尔分数附近大量脂质（如甾醇和含芘酰基链）浓度的微小变化而得到微调。
