大鼠大脑皮层粗突触体组分中[3H]牛磺酸释放的特性。

Properties of [3H]taurine release from crude synaptosomal fractions of rat cerebral cortex.

作者信息

Placheta P, Singer E, Sieghart W, Karobath M

出版信息

Neurochem Res. 1979 Dec;4(6):703-12. doi: 10.1007/BF00964467.

PMID:120936

Abstract

The release of previously accumulated [3H]taurine and [14C]GABA from crude synaptosomal (P2) fractions isolated from rat cerebral cortex was studied using a superfusion system. The spontaneous efflux of [3H]taurine and [14C]GABA was stimulated by elevated concentrations of K+ (15--133 mM) in a concentration-dependent manner. This K+-stimulated relase of [14C]Gaba but not of [3H]taurine was enhanced in the presence of Ca2+. However, addition of 3 mM Ca2+ to the superfusion medium in the presence of the ionophore A 23187 resulted in a stimulation of the release of both [3H]taurine and [14C]GABA. These results are discussed in connection with the cellular localization of taurine in the central nervous system.

摘要

使用灌注系统研究了从大鼠大脑皮层分离的粗制突触体（P2）组分中先前积累的[3H]牛磺酸和[14C]γ-氨基丁酸（GABA）的释放情况。[3H]牛磺酸和[14C]GABA的自发流出受到浓度升高的K +（15 - 133 mM）以浓度依赖性方式刺激。这种K +刺激的[14C]GABA而非[3H]牛磺酸的释放，在Ca2 +存在时增强。然而，在离子载体A 23187存在下向灌注培养基中添加3 mM Ca2 +导致[3H]牛磺酸和[14C]GABA的释放均受到刺激。结合牛磺酸在中枢神经系统中的细胞定位对这些结果进行了讨论。