Holopainen I, Kontro P, Oja S S
Neurochem Res. 1985 Jan;10(1):123-31. doi: 10.1007/BF00964777.
The spontaneous and stimulated release of taurine and hypotaurine from astrocytes in primary cultures were investigated. Spontaneous efflux was slow, less than one half of preloaded labeled taurine and hypotaurine still remaining in the cells after a 60-min efflux period. The release processes of both amino acids were in principle similar. No homo- or heteroexchange with extracellularly added taurine, hypotaurine or GABA could be detected, and depolarizing potassium concentrations failed to stimulate taurine or hypotaurine release. On the other hand, omission of calcium ions from medium increased efflux of taurine and hypotaurine about three- and twofold, respectively, in both high-K+ and normal-K+ media.
对原代培养星形胶质细胞中牛磺酸和次牛磺酸的自发释放及刺激释放进行了研究。自发流出缓慢，在60分钟的流出期后，仍有不到一半预加载的标记牛磺酸和次牛磺酸留在细胞中。两种氨基酸的释放过程原则上相似。未检测到与细胞外添加的牛磺酸、次牛磺酸或GABA的同型或异型交换，去极化钾浓度也未能刺激牛磺酸或次牛磺酸的释放。另一方面，在高钾和正常钾培养基中，从培养基中去除钙离子分别使牛磺酸和次牛磺酸的流出增加约三倍和两倍。
