Dalle Lucca Jurandir J, Saari Jack T, Falcone Jeff C, Schuschke Dale A
Department of Physiology and Biophysics, Health Sciences Center, University of Louisville, Louisville, KY 40292, USA.
Exp Biol Med (Maywood). 2002 Jul;227(7):487-91.
Dietary copper is an essential trace element with roles in both functional and structural aspects of the cardiovascular system. In particular, the vascular response to inflammatory stimuli is known to be significantly augmented in copper-deficient rats. The current study was designed to quantify the extent of injury-induced neointimal proliferation and stenosis in rats fed diets either adequate or deficient in copper. Male, weanling Sprague-Dawley rats were fed purified diets that were either adequate (CuA; 5.6 microg Cu/g) or deficient (CuD; 0.3 microg Cu/g) in copper for 4 weeks. Balloon injury was induced in the left external carotid arteries. Fourteen days after injury, histomorphometric analysis of cross-sections from carotid arteries showed increased neointimal formation in the CuD group compared with the CuA controls (neointima/media ratio: 4.55 +/- 0.93 vs 1.45 +/- 0.2, respectively). These results correspond with data indicating that the activity of Cu/Zn-superoxide dismutase (SOD) is depressed in rats fed this CuD diet. Because superoxide anion and redox status are known to play a key role in the extent of neointimal formation in response to injury, we propose that the exaggerated neointimal proliferation seen in the CuD group is the result of the diminished Cu/Zn-SOD activity.
膳食铜是一种必需的微量元素，在心血管系统的功能和结构方面均发挥作用。特别是，已知缺铜大鼠对炎症刺激的血管反应会显著增强。本研究旨在量化喂食充足或缺乏铜的饮食的大鼠中损伤诱导的新生内膜增殖和狭窄程度。雄性断奶Sprague-Dawley大鼠喂食纯化饮食4周，饮食中的铜含量分别为充足（CuA；5.6微克铜/克）或缺乏（CuD；0.3微克铜/克）。对左颈外动脉进行球囊损伤。损伤后14天，对颈动脉横截面进行组织形态计量学分析，结果显示，与CuA对照组相比，CuD组新生内膜形成增加（新生内膜/中膜比值分别为4.55±0.93和1.45±0.2）。这些结果与以下数据一致，即喂食这种CuD饮食的大鼠中铜/锌超氧化物歧化酶（SOD）的活性受到抑制。由于已知超氧阴离子和氧化还原状态在损伤后新生内膜形成的程度中起关键作用，我们认为在CuD组中看到的新生内膜过度增殖是Cu/锌-SOD活性降低的结果。
