Krishan Awtar, Arya Poonam
Radiation Oncology Department, University of Miami Medical School, Division of Experimental Therapeutics (R-71), P.O. Box 01690, Miami, FL 33101, USA.
Hematol Oncol Clin North Am. 2002 Apr;16(2):357-72, vi. doi: 10.1016/s0889-8588(01)00016-8.
Cellular resistance to a broad spectrum of natural products used as antitumor drugs is believed to be a major cause for the failure of chemotherapy. Flow cytometry has been used for monitoring the expression of drug resistance markers, determining accumulation of fluorescent drugs, and for screening of drugs that enhance chemosensitivity by blocking efflux and enhancing drug retention. This article reviews recent developments in our understanding of the multiple drug resistance phenotype and the use of flow cytometry for the study of drug efflux and its modulation in human tumor cells.
细胞对用作抗肿瘤药物的多种天然产物产生耐药性被认为是化疗失败的主要原因。流式细胞术已被用于监测耐药标志物的表达、确定荧光药物的积累，以及筛选通过阻断外排和增强药物滞留来提高化疗敏感性的药物。本文综述了我们对多药耐药表型的最新认识，以及流式细胞术在研究人类肿瘤细胞中药物外排及其调节方面的应用。
Zotero 插件