蚯蚓表皮胶原蛋白合成的放射自显影研究。

A radioautographic study of collagen synthesis by earthworm epidermis.

作者信息

Burke J M, Ross R

出版信息

Tissue Cell. 1975;7(4):631-50. doi: 10.1016/0040-8166(75)90032-4.

PMID:1209586

Abstract

Secreation by the epidermis of two oligochaetes (Eisenia and Enchytraeus) was investigated radioautographically following administration of 3H-proline, 3H-tryptophan or Na2(35)SO4. Regionally epidermal columnar cells of Enchytraeus synthesize the overlying, probably collagenous, cuticle. Eisenia epidermis does not recordably synthesize the cuticle until after wounding (first eight segments removed). By two days postoperative the epidermal columnar cells of Eisenia synthesize the collagenous cuticle and, later in regeneration, the epidermis may simultaneously synthesize the different collagen of the underlying basement lamella. The epidermis of Enchytraeus, but not of Eisenia, synthesizes some sulfated material associated with the cuticle surface.

摘要

在给予³H - 脯氨酸、³H - 色氨酸或Na₂³⁵SO₄后，通过放射自显影术研究了两种寡毛纲动物（正蚓属和线蚓属）表皮的分泌情况。线蚓属的局部表皮柱状细胞合成覆盖其上的、可能是胶原质的角质层。直到受伤（去除前八个体节）后，正蚓属的表皮才开始有可记录的角质层合成。术后两天，正蚓属的表皮柱状细胞合成胶原质角质层，在再生后期，表皮可能同时合成其下方基底膜的不同胶原蛋白。线蚓属的表皮而非正蚓属的表皮合成一些与角质层表面相关的硫酸化物质。