克隆水稻中一种铁调节金属转运蛋白

Cloning an iron-regulated metal transporter from rice.

作者信息

Bughio Naimatullah, Yamaguchi Hirotaka, Nishizawa Naoko K, Nakanishi Hiromi, Mori Satoshi

机构信息

Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, 113-8657 Tokyo, Japan.

出版信息

J Exp Bot. 2002 Jul;53(374):1677-82. doi: 10.1093/jxb/erf004.

DOI:10.1093/jxb/erf004

PMID:12096107

Abstract

Rice cDNA and genomic libraries were screened in order to clone an Fe(II) transporter gene. A cDNA clone highly homologous to the Arabidopsis Fe(II) transporter gene IRT1 was isolated from Fe-deficient rice roots. The cDNA clone was named OsIRT1. A genomic clone corresponding to the cDNA was also obtained, sequenced and analysed. When expressed in yeast cells, OsIRT1 cDNA reversed the growth defects of the yeast iron-uptake mutant. Northern blot analysis revealed that OsIRT1 mRNA was predominantly expressed in roots and was induced by Fe- and Cu-deficiency. This suggests that OsIRT1 is a functional metal transporter for iron, and is actively engaged in Fe uptake from soils, especially under limiting conditions.

摘要

为了克隆一个亚铁转运蛋白基因，对水稻的cDNA文库和基因组文库进行了筛选。从缺铁的水稻根中分离出一个与拟南芥亚铁转运蛋白基因IRT1高度同源的cDNA克隆。该cDNA克隆被命名为OsIRT1。还获得了与该cDNA对应的基因组克隆，并进行了测序和分析。当在酵母细胞中表达时，OsIRT1 cDNA逆转了酵母铁吸收突变体的生长缺陷。Northern印迹分析表明，OsIRT1 mRNA主要在根中表达，并受缺铁和缺铜诱导。这表明OsIRT1是一种功能性的铁金属转运蛋白，尤其在铁限制条件下，积极参与从土壤中吸收铁。